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recombinant human vegf a165  (MedChemExpress)


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    Structured Review

    MedChemExpress recombinant human vegf a165
    Recombinant Human Vegf A165, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+vegf/VEGF165%2C+Human/pmc13463935-118-5-9
    Average 94 stars, based on 2 article reviews
    recombinant human vegf a165 - by Bioz Stars, 2026-09
    94/100 stars

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    Transfection:

    Article Title: NOL7 Inhibits Ovarian Cancer Progression and Suppresses Angiogenesis by Stabilizing GADD45A to Deactivate STAT3.
    Article Snippet: .. For angiogenesis assays, HUVECs were stimulated with conditioned medium from OVCAR- 3 and SKOV- 3 cells transfected with NOL7 wild type (WT) plasmids for 24 h. For functional validation of VEGF and TSP- 1, HUVECs were treated with conditioned medium from OVCAR- 3 and SKOV- 3 cells, combined with 200 pg/mL recombinant VEGF (rVEGF; MCE, USA) and 20 μg/mL anti- TSP- 1 antibody (Novus Biologicals, USA), for 24 h. For STAT3 inhibitor treatment, OVCAR- 3 cells were exposed to 10 μM of the STAT3 inhibitor HO- 8367 (Aladdin, Shanghai, China) for 24 h [18]. .. NOL7 WT plasmids containing exogenous nuclear localization sequences (NLSs) and NLS mutant plasmids (△NLS23 and △NLS123) [6] were constructed based on the pcDNA3.1 vector and synthesized by Generay Biotechnology (Shanghai, China).

    Functional Assay:

    Article Title: NOL7 Inhibits Ovarian Cancer Progression and Suppresses Angiogenesis by Stabilizing GADD45A to Deactivate STAT3.
    Article Snippet: .. For angiogenesis assays, HUVECs were stimulated with conditioned medium from OVCAR- 3 and SKOV- 3 cells transfected with NOL7 wild type (WT) plasmids for 24 h. For functional validation of VEGF and TSP- 1, HUVECs were treated with conditioned medium from OVCAR- 3 and SKOV- 3 cells, combined with 200 pg/mL recombinant VEGF (rVEGF; MCE, USA) and 20 μg/mL anti- TSP- 1 antibody (Novus Biologicals, USA), for 24 h. For STAT3 inhibitor treatment, OVCAR- 3 cells were exposed to 10 μM of the STAT3 inhibitor HO- 8367 (Aladdin, Shanghai, China) for 24 h [18]. .. NOL7 WT plasmids containing exogenous nuclear localization sequences (NLSs) and NLS mutant plasmids (△NLS23 and △NLS123) [6] were constructed based on the pcDNA3.1 vector and synthesized by Generay Biotechnology (Shanghai, China).

    Biomarker Discovery:

    Article Title: NOL7 Inhibits Ovarian Cancer Progression and Suppresses Angiogenesis by Stabilizing GADD45A to Deactivate STAT3.
    Article Snippet: .. For angiogenesis assays, HUVECs were stimulated with conditioned medium from OVCAR- 3 and SKOV- 3 cells transfected with NOL7 wild type (WT) plasmids for 24 h. For functional validation of VEGF and TSP- 1, HUVECs were treated with conditioned medium from OVCAR- 3 and SKOV- 3 cells, combined with 200 pg/mL recombinant VEGF (rVEGF; MCE, USA) and 20 μg/mL anti- TSP- 1 antibody (Novus Biologicals, USA), for 24 h. For STAT3 inhibitor treatment, OVCAR- 3 cells were exposed to 10 μM of the STAT3 inhibitor HO- 8367 (Aladdin, Shanghai, China) for 24 h [18]. .. NOL7 WT plasmids containing exogenous nuclear localization sequences (NLSs) and NLS mutant plasmids (△NLS23 and △NLS123) [6] were constructed based on the pcDNA3.1 vector and synthesized by Generay Biotechnology (Shanghai, China).

    Recombinant:

    Article Title: NOL7 Inhibits Ovarian Cancer Progression and Suppresses Angiogenesis by Stabilizing GADD45A to Deactivate STAT3.
    Article Snippet: .. For angiogenesis assays, HUVECs were stimulated with conditioned medium from OVCAR- 3 and SKOV- 3 cells transfected with NOL7 wild type (WT) plasmids for 24 h. For functional validation of VEGF and TSP- 1, HUVECs were treated with conditioned medium from OVCAR- 3 and SKOV- 3 cells, combined with 200 pg/mL recombinant VEGF (rVEGF; MCE, USA) and 20 μg/mL anti- TSP- 1 antibody (Novus Biologicals, USA), for 24 h. For STAT3 inhibitor treatment, OVCAR- 3 cells were exposed to 10 μM of the STAT3 inhibitor HO- 8367 (Aladdin, Shanghai, China) for 24 h [18]. .. NOL7 WT plasmids containing exogenous nuclear localization sequences (NLSs) and NLS mutant plasmids (△NLS23 and △NLS123) [6] were constructed based on the pcDNA3.1 vector and synthesized by Generay Biotechnology (Shanghai, China).



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